| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222354 |
| Chromosome: | chromosome 9 |
| Location: | 5234946 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399513 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACATGACCGGAAACTGCACATGTGATGAG |
| Internal bar code: | CGCCGTTCTCACGGCCGACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 97.5034 |
| LEAP-Seq n confirming: | 5663 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCCCACCATCTCTGGA |
| Suggested primer 2: | AAATACGCGCCTTAATGTGG |