| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222357 |
| Chromosome: | chromosome 9 |
| Location: | 2213310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392900 | (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCACCCCCACCCCACCTGGTCAGGTCC |
| Internal bar code: | CGGGCTAGGACCGCGAGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.3171 |
| LEAP-Seq n confirming: | 4654 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTCTGCAGAACTCGAA |
| Suggested primer 2: | CGCCATGTACTCCGTTATCC |