| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222364 |
| Chromosome: | chromosome 12 |
| Location: | 5240451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTGGGGGGCGCCTCATAGAGCTGGGCA |
| Internal bar code: | CGTGCCAGTCGTCTGGACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 99.3143 |
| LEAP-Seq n confirming: | 4490 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGAGTGGTGAAGCAA |
| Suggested primer 2: | ACAGGAGTACGTCGGTGGAC |