| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222403 |
| Chromosome: | chromosome 4 |
| Location: | 495995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217930 | (1 of 1) PF06911 - Senescence-associated protein (Senescence) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGAGCGCTGGCCCGTGTTTGCAGCGC |
| Internal bar code: | CTGGCGTACGATCAGGGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 99.4863 |
| LEAP-Seq n confirming: | 3292 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAATGTCGTGTGTTTGG |
| Suggested primer 2: | AACCCTGCGTACATGGAGTC |