| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222406 |
| Chromosome: | chromosome 4 |
| Location: | 2170054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219750 | HEL22 | (1 of 1) PTHR13710//PTHR13710:SF94 - DNA HELICASE RECQ FAMILY MEMBER // ATP-DEPENDENT DNA HELICASE RECQ FAMILY PROTEIN; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCAGCTGCAGCTCCAGCTCCAGCCCC |
| Internal bar code: | GGGAGTCACTTAAATCTGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 87.3019 |
| LEAP-Seq n confirming: | 3967 |
| LEAP-Seq n nonconfirming: | 577 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCACCACCTGTACCT |
| Suggested primer 2: | AGTTCGTGCTGCAGGTAGGT |