| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222406 |
| Chromosome: | chromosome 4 |
| Location: | 2170064 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219750 | HEL22 | (1 of 1) PTHR13710//PTHR13710:SF94 - DNA HELICASE RECQ FAMILY MEMBER // ATP-DEPENDENT DNA HELICASE RECQ FAMILY PROTEIN; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGTTGGGGTCATGGGGTGTGGATGGGG |
| Internal bar code: | GGGGGGAACCTCTGGATAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 98.7589 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCACCACCTGTACCT |
| Suggested primer 2: | ACTACGGAGGAGGAGGAGGA |