| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222419 |
| Chromosome: | chromosome 2 |
| Location: | 7183570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146300 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCTGCGCTCCCCGCGCGTTCCGCCGCC |
| Internal bar code: | TAATTGGCGTCTCAACGGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 99.6126 |
| LEAP-Seq n confirming: | 12600 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACGTTACCGGCCTTACA |
| Suggested primer 2: | CCAACCCGTCATACGAGACT |