| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222421 |
| Chromosome: | chromosome 16 |
| Location: | 2411510 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660350 | (1 of 1) K15363 - fanconi-associated nuclease 1 (FAN1, MTMR15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACGCGCGCGCACGGTTTCCGCCCTGC |
| Internal bar code: | CGCCGTTACTTCGAAGACTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAGTGCAGCAATGGCAA |
| Suggested primer 2: | CAATGGGTGAGGTGACTGTG |