Insertion junction: LMJ.RY0402.222425_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.222425
Chromosome:chromosome 16
Location:5841048
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g677750 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTGCGCGCGCCGATCGCGTGACACCGG
Internal bar code:GTGGCCGCTCCCGGTCCTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.5067
LEAP-Seq n confirming:4841
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGAGCTTCCTGACACC
Suggested primer 2:GCTCGTAGAAGACCAAACCG