| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222465 |
| Chromosome: | chromosome 7 |
| Location: | 684983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317421 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGCCAAGGCCTTCGCAGCCGCCAGCTC |
| Internal bar code: | ATGTCGCGGGAAGGCCGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 65.2908 |
| LEAP-Seq n confirming: | 348 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCTCCTTGTCCATCAT |
| Suggested primer 2: | AAATGTTGAGCAACTGCACG |