Insertion junction: LMJ.RY0402.222468_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.222468
Chromosome:chromosome 12
Location:3211115
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g498850 CEP12 Cysteine endopeptidase; (1 of 3) 3.4.18.1 - Cathepsin X / Lysosomal carboxypeptidase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACCAACGCTTTGTGTCCCTTTGCTACCC
Internal bar code:CTTCTCCGGGTGTATTAGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:979
LEAP-Seq percent confirming:99.7348
LEAP-Seq n confirming:7145
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTCAGGAAGCCAGACTCG
Suggested primer 2:AGCACATCCCCAAGTACTGC