| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222477 |
| Chromosome: | chromosome 16 |
| Location: | 4411087 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671200 | BLZ20 | bZIP transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTACGGCACCTTCCGAGCAGAGCGGCC |
| Internal bar code: | AGCACCCTGACGCACGGGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 77.9145 |
| LEAP-Seq n confirming: | 4244 |
| LEAP-Seq n nonconfirming: | 1203 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAAAACAACCGGCCTTC |
| Suggested primer 2: | TGTGATGGTGATGGACCCTA |