| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222478 |
| Chromosome: | chromosome_16 |
| Location: | 2350164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g659667 | sense | CDS|intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGTAGATGGCTTCTGGCCGGGTGACCCGT |
| Internal bar code: | ACATCGGGTAGCTAGAATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.5682 |
| LEAP-Seq n confirming: | 9223 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTGGCTGAGCATTTCA |
| Suggested primer 2: | AGTACGGTACATGGCCGAAG |