| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222489 |
| Chromosome: | chromosome 14 |
| Location: | 977019 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614000 | CGL17 | Conserved in the Green Lineage; (1 of 1) PTHR35699:SF1 - F2J10.10 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTGTTTTCGACGTTCCACGTGTGCAGG |
| Internal bar code: | AGCTCCGACCGCCATTCCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 99.4689 |
| LEAP-Seq n confirming: | 6368 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGAGAAGAGGTGGTGCG |
| Suggested primer 2: | TGTAAAGTTGGGAAGGGCAC |