| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222504 |
| Chromosome: | chromosome 17 |
| Location: | 2671184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717700 | (1 of 1) PTHR31833:SF2 - UPF0690 PROTEIN C1ORF52 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGCCTCCCTTACAACCAGCCGCCGCG |
| Internal bar code: | GGGCTATAGGCTGACAGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 99.714 |
| LEAP-Seq n confirming: | 13250 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTTCACATCATCGGTGG |
| Suggested primer 2: | TGTTCTTACTCATGCAGCCG |