| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222516 |
| Chromosome: | chromosome 10 |
| Location: | 1320956 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427600 | KTNA1,KAT1,PF19 | Katanin catalytic subunit, 60 kDa; (1 of 2) PTHR23074:SF19 - KATANIN P60 ATPASE-CONTAINING SUBUNIT A1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAGTGGGCCATGCATGATGCCTGTGTG |
| Internal bar code: | GGTTTTAAGTTTTGCTCTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1199 |
| LEAP-Seq percent confirming: | 99.7615 |
| LEAP-Seq n confirming: | 14224 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCCAGTGAAGAAGTCG |
| Suggested primer 2: | AATACTGTGCCCTGGAATCG |