| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222526 |
| Chromosome: | chromosome 3 |
| Location: | 1404134 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151000 | SEX4,DSP8 | Laforin glucan phosphatase, chloroplastic; (1 of 1) PTHR10343//PTHR10343:SF58 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT // PHOSPHOGLUCAN PHOSPHATASE DSP4, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCACACACACGTGCACAGGCACGAGG |
| Internal bar code: | TGGATGTACCCCCGGAATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.1147 |
| LEAP-Seq n confirming: | 4702 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTACAGACGCAATCCC |
| Suggested primer 2: | TCACATACCCTAAGCTGCCC |