| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222526 |
| Chromosome: | chromosome_9 |
| Location: | 2173785 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g393150 | FOX1 | Multicopper ferroxidase | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGATTACAACCATGGGCTATACCAATGATA |
| Internal bar code: | GAATCACCGGGCCAAAGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 11 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATAATGTACCCGGTTTG |
| Suggested primer 2: | AGCGTCAAGCCAACTCTGAT |