| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222527 |
| Chromosome: | chromosome 2 |
| Location: | 3436070 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095113 | DIV24,TFCE1,TFC-E | Tubulin-folding cofactor subunit; (1 of 1) IPR000938//IPR029071 - CAP Gly-rich domain // Ubiquitin-related domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGAATATGAGAAGGGTGGGGCTGAAAA |
| Internal bar code: | CGGGCTCATGACAGGGCGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.8483 |
| LEAP-Seq n confirming: | 3948 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCACAGGCAGTCTCCA |
| Suggested primer 2: | AACAGGGGTACATTCTTGCG |