| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222541 |
| Chromosome: | chromosome_16 |
| Location: | 1202028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650750 | FAP268 | Flagella Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCTGCAACACGCGGTCCCAGCCACGTCA |
| Internal bar code: | CCTTTTCTCTGTCGTACTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 96.153 |
| LEAP-Seq n confirming: | 4524 |
| LEAP-Seq n nonconfirming: | 181 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGTCAAGGAAGCCAACC |
| Suggested primer 2: | GGAACCACAGCATTGACCTT |