| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222561 |
| Chromosome: | chromosome 2 |
| Location: | 3839383 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095750 | ZEP1,ZXE1 | (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACACCCGCACCGCCCGCATGCATGCA |
| Internal bar code: | GGCGCTGGCACGCTGGATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.7961 |
| LEAP-Seq n confirming: | 1468 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCATGATCAGCCTGGG |
| Suggested primer 2: | AGTTCATGTGGCCCAGGTAG |