Insertion junction: LMJ.RY0402.222564_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.222564
Chromosome:chromosome 9
Location:4617961
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g396549 (1 of 12) IPR001841//IPR002110//IPR020683 - Zinc finger, RING-type // Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCAGCAGACATCAGCCCCCAGCTCAC
Internal bar code:TCGGAGCGGGTCCAGTCCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:99.7963
LEAP-Seq n confirming:2940
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCGCTAGGCTAACTTG
Suggested primer 2:CTTAGCAAGTCGGTTGAGGC