| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222575 |
| Chromosome: | chromosome 8 |
| Location: | 4038425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379300 | (1 of 1) PTHR12459//PTHR12459:SF6 - UNCHARACTERIZED // TRANSMEMBRANE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGCGGGCAGGGACGCAACTCGCAAGC |
| Internal bar code: | GGTCGGTCCACGCCGGTAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 69.677 |
| LEAP-Seq n confirming: | 1057 |
| LEAP-Seq n nonconfirming: | 460 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCGACATTGAGTGTTA |
| Suggested primer 2: | CCAAACGTCAGCACTTCAAA |