Insertion junction: LMJ.RY0402.222575_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.222575
Chromosome:chromosome 8
Location:4038425
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g379300 (1 of 1) PTHR12459//PTHR12459:SF6 - UNCHARACTERIZED // TRANSMEMBRANE PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCGGCGATGCGCCCTCCGGCTGCCATG
Internal bar code:TTGGGCTTCTCCACGCCCCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:2880
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCCGACATTGAGTGTTA
Suggested primer 2:CCAAACGTCAGCACTTCAAA