Insertion junction: LMJ.RY0402.222600_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.222600
Chromosome:chromosome 3
Location:2648699
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g160953 (1 of 1) 3.1.27.1//3.6.3.44 - Ribonuclease T(2) / Ribonuclease T2 // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCAGGTGAGCTGCCCGCGTGTGCGTGTG
Internal bar code:GTGGAGCGCCAGAGGTGCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:229
LEAP-Seq percent confirming:7.87964
LEAP-Seq n confirming:563
LEAP-Seq n nonconfirming:6582
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TACAATTACAGGCGCCACAA
Suggested primer 2:ACGAGGTTGTCCCAAAACAG