| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222604 |
| Chromosome: | chromosome 9 |
| Location: | 4678937 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396809 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTCTGTAACGCTTACGGTGCGGTACTT |
| Internal bar code: | GTTGGGTACACATGTCCGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.3719 |
| LEAP-Seq n confirming: | 3006 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTGGTTTGAGGTAGTGC |
| Suggested primer 2: | AAGAGGATTTGTCCGTGTGG |