| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222619 |
| Chromosome: | chromosome 11 |
| Location: | 833569 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467647 | (1 of 1) K12179 - COP9 signalosome complex subunit 6 (COPS6, CSN6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTAAAATGGAGGTGGATGGCACGCGG |
| Internal bar code: | TTCCGCCTGAGTTCTCGTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 98.3889 |
| LEAP-Seq n confirming: | 8000 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATGTCTAGGGTGCCGT |
| Suggested primer 2: | GCGTTTTGCAGATGTTGCTA |