| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222641 |
| Chromosome: | chromosome 16 |
| Location: | 2539534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661250 | THB9 | (1 of 6) PTHR10681//PTHR10681:SF118 - THIOREDOXIN PEROXIDASE // SUBFAMILY NOT NAMED; Truncated hemoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCACATCGCCGCGTCACGAGCTTGCG |
| Internal bar code: | ATGCTGGTTCGGGCAAGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.3009 |
| LEAP-Seq n confirming: | 4403 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCTTTGACTTCGGCTC |
| Suggested primer 2: | GCCTCATCCAGTTACCTCCA |