| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222664 |
| Chromosome: | chromosome 11 |
| Location: | 3103940 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479000 | CYG7 | (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGTCATGCGAATTGCTATGTGCCGTG |
| Internal bar code: | GTCTCTCAATCCGGGGACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 98.165 |
| LEAP-Seq n confirming: | 4975 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATGGACAGTGTAGCGGA |
| Suggested primer 2: | ACCTTGATTGGCTCCAACTG |