| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222669 |
| Chromosome: | chromosome 12 |
| Location: | 7240423 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558250 | SNR7,TML1 | Regulatory R-SNARE protein, Tomsyn/Sro family (R.Reg); (1 of 1) PTHR10241 - LETHAL 2 GIANT LARVAE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGAGGCAGGGTGTGTGTGGGGATACCA |
| Internal bar code: | TCTAACCTCTTCGTGCCGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 24.8166 |
| LEAP-Seq n confirming: | 778 |
| LEAP-Seq n nonconfirming: | 2357 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACACACTACAGCACCCC |
| Suggested primer 2: | GCTCTTCTTGGTAACTGCGG |