| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222684 |
| Chromosome: | chromosome 3 |
| Location: | 508777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145227 | (1 of 1) K12199 - vacuolar protein sorting-associated protein VTA1 (VTA1, LIP5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAGAGAGCGCAGGAGATTCAGGCAGCC |
| Internal bar code: | GGTAAATATTCAAGAAAGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.6365 |
| LEAP-Seq n confirming: | 6304 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGTTCGCTCAAAGTC |
| Suggested primer 2: | TCTCCAGCTGGTTGAGTGTG |