| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222689 |
| Chromosome: | chromosome 9 |
| Location: | 7238725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGTTGAACTCACCGGCGCCTGCTGTG |
| Internal bar code: | GGTGAGCTCCCGTGCGGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 66.0125 |
| LEAP-Seq n confirming: | 1581 |
| LEAP-Seq n nonconfirming: | 814 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTCGAAACTAACCCCA |
| Suggested primer 2: | CTGCCTCTCTTGCTTGCTCT |