Insertion junction: LMJ.RY0402.222694_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.222694
Chromosome:chromosome 9
Location:1699408
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396950 MFT25,CGL15,PHT4I,PHT4-9 Na+-dependent inorganic phosphate cotransporter; (1 of 4) K08193 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), other (SLC17A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACTGTATCAAGCTCAACGTGTCCCTGA
Internal bar code:TTGTAGTTGAGCGGTAACGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:979
LEAP-Seq percent confirming:99.587
LEAP-Seq n confirming:25078
LEAP-Seq n nonconfirming:104
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCAATGGTGACGAGCTAA
Suggested primer 2:TGAAGGCACAAAGAATGCAG