| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222726 |
| Chromosome: | chromosome 12 |
| Location: | 6894464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560750 | (1 of 10) PF02536 - mTERF (mTERF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACGGATGTTTACCTGGAGTTACCTCGC |
| Internal bar code: | TATGTTTTTTGTTCGTGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 62.3633 |
| LEAP-Seq n confirming: | 3763 |
| LEAP-Seq n nonconfirming: | 2271 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCGTACACTCCGGACAC |
| Suggested primer 2: | CAACATCATCAACCCTGCAC |