| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222775 |
| Chromosome: | chromosome 12 |
| Location: | 4087753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517800 | (1 of 18) PF12906 - RING-variant domain (RINGv) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCAAATGGCAGCCCCTGCTCCATGTA |
| Internal bar code: | GTTCCCGTTACGCGTACGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.7482 |
| LEAP-Seq n confirming: | 2377 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAGGCACTTCAGCATCA |
| Suggested primer 2: | TAACAGGTCCCGTAAGCCAC |