| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222782 |
| Chromosome: | chromosome 15 |
| Location: | 864452 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639503 | GEX173 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGTCTTTGGGGTAGGACTTGGTGTCC |
| Internal bar code: | GTAGGGACGGTTTGGCGCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 71.0526 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTTCGTTGGTTTTGGTT |
| Suggested primer 2: | ATAAGTCTCACCGCCACCAC |