| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222790 |
| Chromosome: | chromosome 6 |
| Location: | 2363741 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267600 | LCYE,LEC1,LCYE1 | (1 of 1) 5.5.1.18 - Lycopene epsilon-cyclase; lycopene epsilon cyclase, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACATTCATGTTCAACCGCCGCTCTCCT |
| Internal bar code: | ACGTGTGACATTCAATCTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.6159 |
| LEAP-Seq n confirming: | 13486 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | ATGGTTTGGGCAGCTTACAC |