| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222791 |
| Chromosome: | chromosome 3 |
| Location: | 2433422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTACCGTATGCTGTATGCCGTAACTCCG |
| Internal bar code: | AATCCACTCGGTTCGACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.0424 |
| LEAP-Seq n confirming: | 724 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTACCAAGAGGTTGCC |
| Suggested primer 2: | ACGCTTTGCAGAGGTGTTCT |