| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222791 |
| Chromosome: | chromosome 17 |
| Location: | 1800502 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709550 | (1 of 2) K06709 - jumonji domain-containing protein 2 [EC:1.14.11.-] (JMJD2, JHDM3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGACATCCCAGTGACGACTAACGGCCTA |
| Internal bar code: | TGATGAAATTCCGCCTCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 99.5751 |
| LEAP-Seq n confirming: | 703 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCGCTTCTTCTACCTGG |
| Suggested primer 2: | GTCTGTCGACAAGAGGAGGC |