| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222803 |
| Chromosome: | chromosome 17 |
| Location: | 7102235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747297 | FAP37 | Flagellar Associated Protein 37; (1 of 1) K04794 - peptidyl-tRNA hydrolase, PTH2 family [EC:3.1.1.29] (PTH2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCCGCAGCACGGCTGCTTCGAGCAGA |
| Internal bar code: | GGTTAAGTCGCTCGCAACTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.4018 |
| LEAP-Seq n confirming: | 2991 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATGGAGGACTGTTAGGG |
| Suggested primer 2: | CTACGGGTAATGAGCGGTGT |