| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222817 |
| Chromosome: | chromosome 13 |
| Location: | 2784474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582800 | (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCTCCTCCACACCCCGCGTGCGGCCC |
| Internal bar code: | CGGTTCGGCAGACCTGCAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 98.9549 |
| LEAP-Seq n confirming: | 9753 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGACTAGGGCTGACGAGC |
| Suggested primer 2: | ACACAGCCGCCTTGTTATTC |