| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222818 |
| Chromosome: | chromosome 12 |
| Location: | 413421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493903 | CSC7 | Chlamydomonadale-specific family C protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGGTACTTACAAATCACCCGAGCTACA |
| Internal bar code: | GTGCCCGATAGGGTTTCCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 99.1561 |
| LEAP-Seq n confirming: | 3760 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATAGTGCTGGGACCCTT |
| Suggested primer 2: | ATCATGTTCGCACACCATGT |