| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222824 |
| Chromosome: | chromosome 11 |
| Location: | 806241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467641 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAATCTACAACCGCCTCTCGCGGAAGGC |
| Internal bar code: | GTCTCGGGCTACAGCTGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 99.56 |
| LEAP-Seq n confirming: | 5430 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCACAGAAAAGGGAC |
| Suggested primer 2: | TACAGTACAGCGGGATGCAG |