| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222856 |
| Chromosome: | chromosome 8 |
| Location: | 36611 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358400 | (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCACGGCCCTATCACAAGGAGGGACA |
| Internal bar code: | GTGTAGGGAGGGATACGACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 88.8676 |
| LEAP-Seq n confirming: | 926 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCACTACGCCCAGATGTT |
| Suggested primer 2: | GTCTACGTCAAGCCCCCATA |