| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222860 |
| Chromosome: | chromosome 1 |
| Location: | 4278352 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028650 | (1 of 726) IPR011009 - Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTCTGACACGCGCTGCCGTTCCAGGGC |
| Internal bar code: | TCTTCGTCCCTTGGTGGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 99.752 |
| LEAP-Seq n confirming: | 6839 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCAGACCTGAAAGAGCC |
| Suggested primer 2: | CGGCACAACAAAAATACGTG |