Insertion junction: LMJ.RY0402.222870_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.222870
Chromosome:chromosome 10
Location:4250046
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g450350 Hspb11,FAP232,IFT25 Intraflagellar Transport Protein 25; (1 of 1) K19369 - heat shock protein beta-11 (HSPB11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTGCGGTGCATGAGCGCGGCAACCTGT
Internal bar code:GTTAGCTCGTCCCGGCCCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:728
LEAP-Seq percent confirming:99.477
LEAP-Seq n confirming:5896
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTACGGGGTCCAATGAC
Suggested primer 2:GACACAGTAGGGGCCACATT