| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222878 |
| Chromosome: | chromosome 14 |
| Location: | 3328362 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629920 | AMT1B,AMT2 | (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP); Ammonium transporter (AMT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGCCGGCGGGTGCGGTGCAGCTGGGG |
| Internal bar code: | GGCCATCCGTGGGCAGCTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.3649 |
| LEAP-Seq n confirming: | 1408 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCACACGCACTAACAAC |
| Suggested primer 2: | AACCACAGCAGTACGGTTCC |