| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222897 |
| Chromosome: | chromosome 12 |
| Location: | 4637673 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGCGACGGTGGGTGGTATAAGTACCGT |
| Internal bar code: | ATGTCGCGGGCATCTGGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 94.7099 |
| LEAP-Seq n confirming: | 1110 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCTTGGGTTGGTCGTTG |
| Suggested primer 2: | GAACTCGAACAAAAGCTCGG |