| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222926 |
| Chromosome: | chromosome 3 |
| Location: | 2267300 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157900 | (1 of 1) PTHR18937//PTHR18937:SF162 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTTCTTGCATTGGTAGTCGCCGGTTA |
| Internal bar code: | TATTTTAGACGGCGGCTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.7204 |
| LEAP-Seq n confirming: | 3567 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGCGACATACCAGGTTA |
| Suggested primer 2: | CAAAATAGCCTTGCCTCTGC |