| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222932 |
| Chromosome: | chromosome 12 |
| Location: | 2750314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCTCGTGCTGCTGCCAGTATTCCCGT |
| Internal bar code: | GGATGGTGAGCATATGGAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 97.3404 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTGGTTTGCTTAGCTGC |
| Suggested primer 2: | CGCACTCTCACAAACACCAT |